Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs

CRISPR-Cas9 nucleases are powerful genome engineering tools, but unwanted cleavage at off-target and previously edited sites remains a major concern. Numerous strategies to reduce unwanted cleavage have been devised, but all are imperfect. Here, we report that off-target sites can be shielded from t...

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Publicado en:Nat Commun
Main Authors: Rose, John C., Popp, Nicholas A., Richardson, Christopher D., Stephany, Jason J., Mathieu, Julie, Wei, Cindy T., Corn, Jacob E., Maly, Dustin J., Fowler, Douglas M.
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2020
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7264211/
https://ncbi.nlm.nih.gov/pubmed/32483117
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41467-020-16542-9
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