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NOseq: amplicon sequencing evaluation method for RNA m(6)A sites after chemical deamination
Methods for the detection of m(6)A by RNA-Seq technologies are increasingly sought after. We here present NOseq, a method to detect m(6)A residues in defined amplicons by virtue of their resistance to chemical deamination, effected by nitrous acid. Partial deamination in NOseq affects all exocyclic...
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| Publicat a: | Nucleic Acids Res |
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| Autors principals: | , , , , , , , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Oxford University Press
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7913672/ https://ncbi.nlm.nih.gov/pubmed/33313868 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1173 |
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