NOseq: amplicon sequencing evaluation method for RNA m(6)A sites after chemical deamination

Methods for the detection of m(6)A by RNA-Seq technologies are increasingly sought after. We here present NOseq, a method to detect m(6)A residues in defined amplicons by virtue of their resistance to chemical deamination, effected by nitrous acid. Partial deamination in NOseq affects all exocyclic...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Werner, Stephan, Galliot, Aurellia, Pichot, Florian, Kemmer, Thomas, Marchand, Virginie, Sednev, Maksim V, Lence, Tina, Roignant, Jean-Yves, König, Julian, Höbartner, Claudia, Motorin, Yuri, Hildebrandt, Andreas, Helm, Mark
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Methods Online
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7913672/
https://ncbi.nlm.nih.gov/pubmed/33313868
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa1173
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