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Laboratory Evolution of a Sortase Enzyme that Modifies Amyloid β-protein
Epitope-specific enzymes are powerful tools for site-specific protein modification, but generally require genetic manipulation of the target protein. Here, we describe the laboratory evolution of the bacterial transpeptidase sortase A to recognize the LMVGG sequence in endogenous Aβ protein. Using a...
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| Udgivet i: | Nat Chem Biol |
|---|---|
| Main Authors: | , , , , , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2021
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7904614/ https://ncbi.nlm.nih.gov/pubmed/33432237 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41589-020-00706-1 |
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