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A general strategy for the evolution of bond-forming enzymes using yeast display
The ability to routinely generate efficient protein catalysts of bond-forming reactions chosen by researchers, rather than nature, is a long-standing goal of the molecular life sciences. Here, we describe a directed evolution strategy for enzymes that catalyze, in principle, any bond-forming reactio...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2011
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3136257/ https://ncbi.nlm.nih.gov/pubmed/21697512 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1101046108 |
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