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A general strategy for the evolution of bond-forming enzymes using yeast display

The ability to routinely generate efficient protein catalysts of bond-forming reactions chosen by researchers, rather than nature, is a long-standing goal of the molecular life sciences. Here, we describe a directed evolution strategy for enzymes that catalyze, in principle, any bond-forming reactio...

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Autors principals: Chen, Irwin, Dorr, Brent M., Liu, David R.
Format: Artigo
Idioma:Inglês
Publicat: National Academy of Sciences 2011
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3136257/
https://ncbi.nlm.nih.gov/pubmed/21697512
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1101046108
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