Expansion Sequencing: Spatially Precise In Situ Transcriptomics in Intact Biological Systems

Methods for highly multiplexed RNA imaging are limited in spatial resolution, and thus in their ability to localize transcripts to nanoscale and subcellular compartments. We adapt expansion microscopy, which physically expands biological specimens, for long-read untargeted and targeted in situ RNA s...

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Detaylı Bibliyografya
Yayımlandı:Science
Asıl Yazarlar: Alon, Shahar, Goodwin, Daniel R, Sinha, Anubhav, Wassie, Asmamaw T, Chen, Fei, Daugharthy, Evan R, Bando, Yosuke, Kajita, Atsushi, Xue, Andrew G, Marrett, Karl, Prior, Robert, Cui, Yi, Payne, Andrew C, Yao, Chun-Chen, Suk, Ho-Jun, Wang, Ru, Yu, Chih-Chieh (Jay), Tillberg, Paul, Reginato, Paul, Pak, Nikita, Liu, Songlei, Punthambaker, Sukanya, Iyer, Eswar P. R., Kohman, Richie E, Miller, Jeremy A, Lein, Ed S, Lako, Ana, Cullen, Nicole, Rodig, Scott, Helvie, Karla, Abravanel, Daniel L, Wagle, Nikhil, Johnson, Bruce E, Klughammer, Johanna, Slyper, Michal, Waldman, Julia, Jané-Valbuena, Judit, Rozenblatt-Rosen, Orit, Regev, Aviv, Church, George M, Marblestone, Adam H, Boyden, Edward S
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: 2021
Konular:
Article
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC7900882/
https://ncbi.nlm.nih.gov/pubmed/33509999
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1126/science.aax2656
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