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An optimized workflow for CRISPR-Cas9 deletion of surface and intracellular factors in primary human T lymphocytes

The appropriate regulation of T lymphocyte functions is key to achieve protective immune responses, while at the same time limiting the risks of tissue damage and chronic inflammation. Deciphering the mechanisms underpinning T cell responses in humans may therefore be beneficial for a range of infec...

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Bibliografiske detaljer
Udgivet i:PLoS One
Main Authors: Leoni, Cristina, Bianchi, Niccolò, Vincenzetti, Lucia, Monticelli, Silvia
Format: Artigo
Sprog:Inglês
Udgivet: Public Library of Science 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7891767/
https://ncbi.nlm.nih.gov/pubmed/33600503
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0247232
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