Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In our experiments, we targeted two different ge...

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Bibliografiska uppgifter
I publikationen:Front Plant Sci
Huvudupphovsmän: Stukenberg, Daniel, Zauner, Stefan, Dell’Aquila, Gianluca, Maier, Uwe G.
Materialtyp: Artigo
Språk:Inglês
Publicerad: Frontiers Media S.A. 2018
Ämnen:
Plant Science
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC5998643/
https://ncbi.nlm.nih.gov/pubmed/29928285
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2018.00740
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