Efficient CRISPR/Cas9 mediated Pooled-sgRNAs assembly accelerates targeting multiple genes related to male sterility in cotton

BACKGROUND: Upland cotton (Gossypium hirsutum), harboring a complex allotetraploid genome, consists of A and D sub-genomes. Every gene has multiple copies with high sequence similarity that makes genetic, genomic and functional analyses extremely challenging. The recent accessibility of CRISPR/Cas9...

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Publicat a:Plant Methods
Autors principals: Ramadan, Mohamed, Alariqi, Muna, Ma, Yizan, Li, Yanlong, Liu, Zhenping, Zhang, Rui, Jin, Shuangxia, Min, Ling, Zhang, Xianlong
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2021
Matèries:
Methodology
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7869495/
https://ncbi.nlm.nih.gov/pubmed/33557889
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s13007-021-00712-x
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