Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats

CRISPR-Cas9 are widely used for gene targeting in mice and rats. The non-homologous end-joining (NHEJ) repair pathway, which is dominant in zygotes, efficiently induces insertion or deletion (indel) mutations as gene knockouts at targeted sites, whereas gene knock-ins (KIs) via homology-directed rep...

ver descrição completa

Na minha lista:
Detalhes bibliográficos
Publicado no:Hum Genet
Main Authors: Yoshimi, Kazuto, Oka, Yuichiro, Miyasaka, Yoshiki, Kotani, Yuko, Yasumura, Misato, Uno, Yoshihiro, Hattori, Kosuke, Tanigawa, Arisa, Sato, Makoto, Oya, Manami, Nakamura, Kazuhiro, Matsushita, Natsuki, Kobayashi, Kazuto, Mashimo, Tomoji
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Berlin Heidelberg 2020
Assuntos:
Original Investigation
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7864826/
https://ncbi.nlm.nih.gov/pubmed/32617796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00439-020-02198-4
Tags: Adicionar Tag
Sem tags, seja o primeiro a adicionar uma tag!

Registos relacionados