Combi-CRISPR: combination of NHEJ and HDR provides efficient and precise plasmid-based knock-ins in mice and rats

CRISPR-Cas9 are widely used for gene targeting in mice and rats. The non-homologous end-joining (NHEJ) repair pathway, which is dominant in zygotes, efficiently induces insertion or deletion (indel) mutations as gene knockouts at targeted sites, whereas gene knock-ins (KIs) via homology-directed rep...

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Dettagli Bibliografici
Pubblicato in:Hum Genet
Autori principali: Yoshimi, Kazuto, Oka, Yuichiro, Miyasaka, Yoshiki, Kotani, Yuko, Yasumura, Misato, Uno, Yoshihiro, Hattori, Kosuke, Tanigawa, Arisa, Sato, Makoto, Oya, Manami, Nakamura, Kazuhiro, Matsushita, Natsuki, Kobayashi, Kazuto, Mashimo, Tomoji
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer Berlin Heidelberg 2020
Soggetti:
Original Investigation
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7864826/
https://ncbi.nlm.nih.gov/pubmed/32617796
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00439-020-02198-4
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