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Mutation of an atypical oxirane oxyanion hole improves regioselectivity of the α/β-fold epoxide hydrolase Alp1U

Epoxide hydrolases (EHs) have been characterized and engineered as biocatalysts that convert epoxides to valuable chiral vicinal diol precursors of drugs and bioactive compounds. Nonetheless, the regioselectivity control of the epoxide ring opening by EHs remains challenging. Alp1U is an α/β-fold EH...

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Detalhes bibliográficos
Publicado no:J Biol Chem
Main Authors: Zhang, Liping, De, Bidhan Chandra, Zhang, Wenjun, Mándi, Attila, Fang, Zhuangjie, Yang, Chunfang, Zhu, Yiguang, Kurtán, Tibor, Zhang, Changsheng
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7863881/
https://ncbi.nlm.nih.gov/pubmed/33004437
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.RA120.015563
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