Ex vivo assay to evaluate the efficacy of drugs targeting sphingolipids in preventing SARS-CoV-2 infection of nasal epithelial cells

This protocol enables the testing of drugs against infection of epithelial cells with SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2), using pseudo-typed replication deficient vesicular stomatitis virus particles (pp-VSV) presenting the SARS-CoV-2 spike protein. After treating human vol...

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Sparad:
Bibliografiska uppgifter
I publikationen:STAR Protoc
Huvudupphovsmän: Becker, Katrin Anne, Carpinteiro, Alexander, Hoffmann, Markus, Pöhlmann, Stefan, Kornhuber, Johannes, Gulbins, Erich
Materialtyp: Artigo
Språk:Inglês
Publicerad: Elsevier 2021
Ämnen:
Protocol
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC7857112/
https://ncbi.nlm.nih.gov/pubmed/33558862
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100356
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