Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry

Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and highly sensitive mass spectrometric assay for drug-targe...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Cyhoeddwyd yn:Bio Protoc
Prif Awduron: Suvarna, Kruthi, Honda, Kaori, Muroi, Makoto, Kondoh, Yasumitsu, Watanabe, Nobumoto, Osada, Hiroyuki
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: Bio-Protocol 2020
Pynciau:
Methods Article
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842831/
https://ncbi.nlm.nih.gov/pubmed/33654742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3517
Tagiau: Ychwanegu Tag
Dim Tagiau, Byddwch y cyntaf i dagio'r cofnod hwn!

Eitemau Tebyg