Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry

Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and highly sensitive mass spectrometric assay for drug-targe...

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Библиографические подробности
Опубликовано в: :Bio Protoc
Главные авторы: Suvarna, Kruthi, Honda, Kaori, Muroi, Makoto, Kondoh, Yasumitsu, Watanabe, Nobumoto, Osada, Hiroyuki
Формат: Artigo
Язык:Inglês
Опубликовано: Bio-Protocol 2020
Предметы:
Methods Article
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842831/
https://ncbi.nlm.nih.gov/pubmed/33654742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3517
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