Identification of Target Protein for Bio-active Small Molecule Using Photo-cross Linked Beads and MALDI-TOF Mass Spectrometry

Development of methods for protein identification is one of the important aspects of proteomics. Here, we report a protocol for the preparation of compound conjugated beads by photo-crosslinking, affinity purification, gel electrophoresis, and highly sensitive mass spectrometric assay for drug-targe...

Descripción completa

Guardado en:
Detalles Bibliográficos
Publicado en:Bio Protoc
Autores principales: Suvarna, Kruthi, Honda, Kaori, Muroi, Makoto, Kondoh, Yasumitsu, Watanabe, Nobumoto, Osada, Hiroyuki
Formato: Artigo
Lenguaje:Inglês
Publicado: Bio-Protocol 2020
Materias:
Methods Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842831/
https://ncbi.nlm.nih.gov/pubmed/33654742
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3517
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!

Ejemplares similares