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Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for single-cell analysis as a further application. This pr...
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| Udgivet i: | Bio Protoc |
|---|---|
| Main Authors: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Bio-Protocol
2020
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7842824/ https://ncbi.nlm.nih.gov/pubmed/33659432 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3775 |
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