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A Radioactive-free Kinase Inhibitor Discovery Assay Against the Trypanosoma brucei Glycogen Synthase Kinase-3 short (TbGSK-3s)

The identification of small molecules possessing inhibitory activity in vitro, against a given target kinase, is the first step in the drug discovery process. Herein, we describe a non radioactive protocol using luciferase-based ATP assay for the identification of inhibitors for the short isoform of...

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Publicat a:Bio Protoc
Autors principals: Efstathiou, Antonia, Smirlis, Despina
Format: Artigo
Idioma:Inglês
Publicat: Bio-Protocol 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842726/
https://ncbi.nlm.nih.gov/pubmed/33654724
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3493
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