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Quantitative Irreversible Tethering (qIT) for Target-directed Covalent Fragment Screening

Small molecules that react to form covalent bonds with proteins are widely used as biological tools and therapeutic agents. Screening cysteine-reactive fragments against a protein target is an efficient way to identify chemical starting points for covalent probe development. Mass spectrometry is oft...

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Detalhes bibliográficos
Publicado no:Bio Protoc
Main Authors: Craven, Gregory B., Armstrong, Alan, Mann, David J.
Formato: Artigo
Idioma:Inglês
Publicado em: Bio-Protocol 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7842680/
https://ncbi.nlm.nih.gov/pubmed/33659502
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.21769/BioProtoc.3855
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