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An optimized protocol for acquiring and processing cryo-EM data of human 26S proteasome with M1-Ub(6)
The 26S proteasome is specialized for regulated protein degradation. It is formed by a regulatory particle (RP) that recognizes ubiquitinated substrates and caps a hollow cylindrical core particle (CP) where substrates are proteolyzed. Structural heterogeneity caused by dynamics makes it challenging...
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| Vydáno v: | STAR Protoc |
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| Hlavní autoři: | , , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
Elsevier
2021
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7820132/ https://ncbi.nlm.nih.gov/pubmed/33521680 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100278 |
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