Endogenous zebrafish proneural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration

We previously reported efficient precision targeted integration of reporter DNA in zebrafish and human cells using CRISPR/Cas9 and short regions of homology. Here, we apply this strategy to isolate zebrafish Cre recombinase drivers whose spatial and temporal restricted expression mimics endogenous g...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Almeida, Maira P., Welker, Jordan M., Siddiqui, Sahiba, Luiken, Jon, Ekker, Stephen C., Clark, Karl J., Essner, Jeffrey J., McGrail, Maura
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group UK 2021
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7813866/
https://ncbi.nlm.nih.gov/pubmed/33462297
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-021-81239-y
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