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Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice
In the present study, we generated novel cre driver mice for gene manipulation in pancreatic β cells. Using the CRISPR/Cas9 system, stop codon sequences of Ins1 were targeted for insertion of cre, including 2A sequences. A founder of C57BL/6J-Ins1(em1 (cre) Utr) strain was produced from an oocyte in...
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Publicado no: | Exp Anim |
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Main Authors: | , , , , , , , , , , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Japanese Association for Laboratory Animal Science
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4976246/ https://ncbi.nlm.nih.gov/pubmed/27053096 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1538/expanim.16-0016 |
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