Generation of CRISPR/Cas9-mediated bicistronic knock-in ins1-cre driver mice

In the present study, we generated novel cre driver mice for gene manipulation in pancreatic β cells. Using the CRISPR/Cas9 system, stop codon sequences of Ins1 were targeted for insertion of cre, including 2A sequences. A founder of C57BL/6J-Ins1(em1 (cre) Utr) strain was produced from an oocyte in...

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Detalles Bibliográficos
Publicado en:Exp Anim
Main Authors: Hasegawa, Yoshikazu, Hoshino, Yoshikazu, Ibrahim, Abdelaziz E., Kato, Kanako, Daitoku, Yoko, Tanimoto, Yoko, Ikeda, Yoshihisa, Oishi, Hisashi, Takahashi, Satoru, Yoshiki, Atsushi, Yagami, Ken-ichi, Iseki, Hiroyoshi, Mizuno, Seiya, Sugiyama, Fumihiro
Formato: Artigo
Idioma:Inglês
Publicado: Japanese Association for Laboratory Animal Science 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC4976246/
https://ncbi.nlm.nih.gov/pubmed/27053096
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1538/expanim.16-0016
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