Efficient targeted integration directed by short homology in zebrafish and mammalian cells

Efficient precision genome engineering requires high frequency and specificity of integration at the genomic target site. Here, we describe a set of resources to streamline reporter gene knock-ins in zebrafish and demonstrate the broader utility of the method in mammalian cells. Our approach uses sh...

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Detalhes bibliográficos
Publicado no:eLife
Main Authors: Wierson, Wesley A, Welker, Jordan M, Almeida, Maira P, Mann, Carla M, Webster, Dennis A, Torrie, Melanie E, Weiss, Trevor J, Kambakam, Sekhar, Vollbrecht, Macy K, Lan, Merrina, McKeighan, Kenna C, Levey, Jacklyn, Ming, Zhitao, Wehmeier, Alec, Mikelson, Christopher S, Haltom, Jeffrey A, Kwan, Kristen M, Chien, Chi-Bin, Balciunas, Darius, Ekker, Stephen C, Clark, Karl J, Webber, Beau R, Moriarity, Branden S, Solin, Stacy L, Carlson, Daniel F, Dobbs, Drena L, McGrail, Maura, Essner, Jeffrey
Formato: Artigo
Idioma:Inglês
Publicado em: eLife Sciences Publications, Ltd 2020
Assuntos:
Developmental Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7228771/
https://ncbi.nlm.nih.gov/pubmed/32412410
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7554/eLife.53968
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