Expanding the CRISPR Toolbox with ErCas12a in Zebrafish and Human Cells

CRISPR and CRISPR-Cas effector proteins enable the targeting of DNA double-strand breaks to defined loci based on a variable length RNA guide specific to each effector. The guide RNAs are generally similar in size and form, consisting of a ∼20 nucleotide sequence complementary to the DNA target and...

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Detalhes bibliográficos
Publicado no:CRISPR J
Main Authors: Wierson, Wesley A., Simone, Brandon W., WareJoncas, Zachary, Mann, Carla, Welker, Jordan M., Kar, Bibekananda, Emch, Michael J., Friedberg, Iddo, Gendron, William A.C., Barry, Michael A., Clark, Karl J., Dobbs, Drena L., McGrail, Maura A., Ekker, Stephen C., Essner, Jeffrey J.
Formato: Artigo
Idioma:Inglês
Publicado em: Mary Ann Liebert, Inc., publishers 2019
Assuntos:
Research Articles
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC6919245/
https://ncbi.nlm.nih.gov/pubmed/31742435
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2019.0026
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