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Experimental determination of the bioluminescence resonance energy transfer (BRET) Förster distances of NanoBRET and red-shifted BRET pairs

Bioluminescence Resonance Energy Transfer (BRET) is widely applied to study protein-protein interactions, as well as increasingly to monitor both ligand binding and molecular rearrangements. The Förster distance (R(0)) describes the physical distance between the two chromophores at which 50% of the...

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Pubblicato in:Anal Chim Acta X
Autori principali: Weihs, Felix, Wang, Jian, Pfleger, Kevin D.G., Dacres, Helen
Natura: Artigo
Lingua:Inglês
Pubblicazione: Elsevier 2020
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC7772631/
https://ncbi.nlm.nih.gov/pubmed/33392495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.acax.2020.100059
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