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Using nanoBRET and CRISPR/Cas9 to monitor proximity to a genome-edited protein in real-time
Bioluminescence resonance energy transfer (BRET) has been a vital tool for understanding G protein-coupled receptor (GPCR) function. It has been used to investigate GPCR-protein and/or -ligand interactions as well as GPCR oligomerisation. However the utility of BRET is limited by the requirement tha...
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| 出版年: | Sci Rep |
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| 主要な著者: | , , , , |
| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
Nature Publishing Group UK
2017
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5466623/ https://ncbi.nlm.nih.gov/pubmed/28600500 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-017-03486-2 |
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