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An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification

Green fluorescent proteins (GFPs) are widely used to monitor membrane protein expression, purification, and stability. An ideal reporter should be stable itself and provide high sensitivity and yield. Here, we demonstrate that a coral (Galaxea fascicularis) thermostable GFP (TGP) is by such reasons...

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Detalles Bibliográficos
Publicado en:Commun Biol
Main Authors: Cai, Hongmin, Yao, Hebang, Li, Tingting, Hutter, Cedric A. J., Li, Yanfang, Tang, Yannan, Seeger, Markus A., Li, Dianfan
Formato: Artigo
Idioma:Inglês
Publicado: Nature Publishing Group UK 2020
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC7729955/
https://ncbi.nlm.nih.gov/pubmed/33303987
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s42003-020-01478-z
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