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Spin-labeled nanobodies as protein conformational reporters for electron paramagnetic resonance in cellular membranes
Nanobodies are emerging tools in a variety of fields such as structural biology, cell imaging, and drug discovery. Here we pioneer the use of their spin-labeled variants as reporters of conformational dynamics of membrane proteins using DEER spectroscopy. At the example of the bacterial ABC transpor...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2020
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7007536/ https://ncbi.nlm.nih.gov/pubmed/31964841 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1913737117 |
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