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Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins

Advanced fluorescence microscopy studies require specific and monovalent molecular labeling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labeled nanobodies against commonly employed fluorescent proteins (FPs). However, very little is known how t...

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Bibliografische gegevens
Gepubliceerd in:iScience
Hoofdauteurs: Schneider, Falk, Sych, Taras, Eggeling, Christian, Sezgin, Erdinc
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2020
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7753935/
https://ncbi.nlm.nih.gov/pubmed/33364580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2020.101891
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