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Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins

Advanced fluorescence microscopy studies require specific and monovalent molecular labeling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labeled nanobodies against commonly employed fluorescent proteins (FPs). However, very little is known how t...

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Detalhes bibliográficos
Publicado no:iScience
Main Authors: Schneider, Falk, Sych, Taras, Eggeling, Christian, Sezgin, Erdinc
Formato: Artigo
Idioma:Inglês
Publicado em: Elsevier 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7753935/
https://ncbi.nlm.nih.gov/pubmed/33364580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2020.101891
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