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Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins

Advanced fluorescence microscopy studies require specific and monovalent molecular labeling with bright and photostable fluorophores. This necessity led to the widespread use of fluorescently labeled nanobodies against commonly employed fluorescent proteins (FPs). However, very little is known how t...

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Библиографические подробности
Опубликовано в: :iScience
Главные авторы: Schneider, Falk, Sych, Taras, Eggeling, Christian, Sezgin, Erdinc
Формат: Artigo
Язык:Inglês
Опубликовано: Elsevier 2020
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Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC7753935/
https://ncbi.nlm.nih.gov/pubmed/33364580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.isci.2020.101891
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