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Reducing Agent‐Mediated Nonenzymatic Conversion of 2‐Oxoglutarate to Succinate: Implications for Oxygenase Assays

l‐Ascorbate (l‐Asc) is often added to assays with isolated Fe(II)‐ and 2‐oxoglutarate (2OG)‐dependent oxygenases to enhance activity. l‐Asc is proposed to be important in catalysis by some 2OG oxygenases in vivo. We report observations on the nonenzymatic conversion of 2OG to succinate, which is med...

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Publicat a:Chembiochem
Autors principals: Khan, Amjad, Schofield, Christopher J., Claridge, Timothy D. W.
Format: Artigo
Idioma:Inglês
Publicat: John Wiley and Sons Inc. 2020
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC7693218/
https://ncbi.nlm.nih.gov/pubmed/32478965
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/cbic.202000185
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