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A low-bias and sensitive small RNA library preparation method using randomized splint ligation

Small RNAs are important regulators of gene expression and are involved in human development and disease. Next generation sequencing (NGS) allows for scalable, genome-wide studies of small RNA; however, current methods are challenged by low sensitivity and high bias, limiting their ability to captur...

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Detalhes bibliográficos
Publicado no:Nucleic Acids Res
Main Authors: Maguire, Sean, Lohman, Gregory J S, Guan, Shengxi
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2020
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7641310/
https://ncbi.nlm.nih.gov/pubmed/32496547
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkaa480
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