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Optimizing splinted ligation of highly structured small RNAs
The synthesis of highly structured small RNAs containing nonstandard nucleotides is of high interest for structural and functional investigations. A general approach is the joining, by T4 DNA ligase-mediated splinted ligation, of two or more RNA fragments, each of which may contain its own set of mo...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Copyright 2005 by RNA Society
2005
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370878/ https://ncbi.nlm.nih.gov/pubmed/16251384 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2170705 |
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