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A general method to quantify ligand-driven oligomerization from fluorescence-based images

Here we introduce fluorescence intensity fluctuation spectrometry for determining the identity, abundance, and stability of protein oligomers. This approach was tested on monomers and oligomers of known sizes and was used to uncover the oligomeric states of the epidermal growth factor receptor and t...

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Detalhes bibliográficos
Publicado no:Nat Methods
Main Authors: Stoneman, Michael R., Biener, Gabriel, Ward, Richard J., Pediani, John D., Badu, Dammar, Eis, Annie, Popa, Ionel, Milligan, Graeme, Raicu, Valerică
Formato: Artigo
Idioma:Inglês
Publicado em: 2019
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7617210/
https://ncbi.nlm.nih.gov/pubmed/31110281
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0408-9
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