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A general method to quantify ligand-driven oligomerization from fluorescence-based images
Here we introduce fluorescence intensity fluctuation spectrometry for determining the identity, abundance, and stability of protein oligomers. This approach was tested on monomers and oligomers of known sizes and was used to uncover the oligomeric states of the epidermal growth factor receptor and t...
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| Publié dans: | Nat Methods |
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| Auteurs principaux: | , , , , , , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2019
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7617210/ https://ncbi.nlm.nih.gov/pubmed/31110281 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41592-019-0408-9 |
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