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Two-photon cell and tissue level laser ablation methods to study morphogenetic biomechanics

Laser ablation is routinely performed to infer mechanical tension in cells and tissues. Here we describe our method of two-photon laser ablation at the cellular and tissue level in mouse embryos. The primary outcome of these experiments is initial retraction following ablation, which correlates with...

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Detalhes bibliográficos
Publicado no:Methods Mol Biol
Main Authors: Marshall, Abigail R, Maniou, Eirini, Moulding, Dale, Greene, Nicholas DE, Copp, Andrew J, Galea, Gabriel L
Formato: Artigo
Idioma:Inglês
Publicado em: 2022
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC7614166/
https://ncbi.nlm.nih.gov/pubmed/35147945
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-2035-9_14
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