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Two-photon cell and tissue level laser ablation methods to study morphogenetic biomechanics

Laser ablation is routinely performed to infer mechanical tension in cells and tissues. Here we describe our method of two-photon laser ablation at the cellular and tissue level in mouse embryos. The primary outcome of these experiments is initial retraction following ablation, which correlates with...

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Détails bibliographiques
Publié dans:Methods Mol Biol
Auteurs principaux: Marshall, Abigail R, Maniou, Eirini, Moulding, Dale, Greene, Nicholas DE, Copp, Andrew J, Galea, Gabriel L
Format: Artigo
Langue:Inglês
Publié: 2022
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7614166/
https://ncbi.nlm.nih.gov/pubmed/35147945
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-0716-2035-9_14
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