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A 68-codon genetic code to incorporate four distinct non-canonical amino acids enabled by automated orthogonal mRNA design

Orthogonal (O-) ribosome mediated translation of O-mRNAs enables the incorporation of up to three distinct non-canonical amino acids (ncAAs) into proteins in Escherichia coli. However, the general and efficient incorporation of multiple distinct ncAAs by O-ribosomes requires scalable strategies for...

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Udgivet i:Nat Chem
Main Authors: Dunkelmann, Daniel L., Oehm, Sebastian B., Beattie, Adam T., Chin, Jason W.
Format: Artigo
Sprog:Inglês
Udgivet: 2021
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7612796/
https://ncbi.nlm.nih.gov/pubmed/34426682
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41557-021-00764-5
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