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Reinitiation at non-canonical start codons leads to leak expression when incorporating unnatural amino acids

With the rapid development of a continuously growing selection of unnatural amino acids (UAAs), UAA insertion becomes increasingly popular for investigating proteins. However, it can prove problematic to ensure the homogeneity of the expressed proteins, when homogeneity is compromised by “leak expre...

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Kaydedildi:
Detaylı Bibliyografya
Yayımlandı:Sci Rep
Asıl Yazarlar: Kalstrup, Tanja, Blunck, Rikard
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: Nature Publishing Group 2015
Konular:
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC4648390/
https://ncbi.nlm.nih.gov/pubmed/26153354
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep11866
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