A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning

ABSTRACT: Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-gui...

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Vydáno v:Appl Microbiol Biotechnol
Hlavní autoři: Schüller, Andreas, Wolansky, Lisa, Berger, Harald, Studt, Lena, Gacek-Matthews, Agnieszka, Sulyok, Michael, Strauss, Joseph
Médium: Artigo
Jazyk:Inglês
Vydáno: Springer Berlin Heidelberg 2020
Témata:
Methods and Protocols
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC7595996/
https://ncbi.nlm.nih.gov/pubmed/33006690
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-020-10900-9
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