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A novel fungal gene regulation system based on inducible VPR-dCas9 and nucleosome map-guided sgRNA positioning
ABSTRACT: Programmable transcriptional regulation is a powerful tool to study gene functions. Current methods to selectively regulate target genes are mainly based on promoter exchange or on overexpressing transcriptional activators. To expand the discovery toolbox, we designed a dCas9-based RNA-gui...
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| Publicat a: | Appl Microbiol Biotechnol |
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| Autors principals: | , , , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Springer Berlin Heidelberg
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7595996/ https://ncbi.nlm.nih.gov/pubmed/33006690 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00253-020-10900-9 |
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