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Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids

In protein engineering and synthetic biology, Methanosarcina mazei pyrrolysyl-tRNA synthetase (MmPylRS), with its cognate tRNA(Pyl), is one of the most popular tools for site-specific incorporation of non-canonical amino acids (ncAAs). Numerous orthogonal pairs based on engineered MmPylRS variants h...

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Bibliografiske detaljer
Udgivet i:Molecules
Main Authors: Tseng, Hsueh-Wei, Baumann, Tobias, Sun, Huan, Wang, Yane-Shih, Ignatova, Zoya, Budisa, Nediljko
Format: Artigo
Sprog:Inglês
Udgivet: MDPI 2020
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7582959/
https://ncbi.nlm.nih.gov/pubmed/32992991
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3390/molecules25194418
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