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Protocol for Titrating Gene Expression Levels in Budding Yeast
The biological phenotype is affected by the level of gene expression. Here, we provide a step-by-step protocol for precisely titrating and quantitatively observing the target gene expression level in budding yeast by manipulating its copy number in the genome. Using this method, we construct various...
Guardat en:
| Publicat a: | STAR Protoc |
|---|---|
| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Elsevier
2020
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7580245/ https://ncbi.nlm.nih.gov/pubmed/33111115 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100082 |
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