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Protocol for Titrating Gene Expression Levels in Budding Yeast
The biological phenotype is affected by the level of gene expression. Here, we provide a step-by-step protocol for precisely titrating and quantitatively observing the target gene expression level in budding yeast by manipulating its copy number in the genome. Using this method, we construct various...
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| Publié dans: | STAR Protoc |
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| Auteurs principaux: | , , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2020
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7580245/ https://ncbi.nlm.nih.gov/pubmed/33111115 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100082 |
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