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Protocol for Titrating Gene Expression Levels in Budding Yeast

The biological phenotype is affected by the level of gene expression. Here, we provide a step-by-step protocol for precisely titrating and quantitatively observing the target gene expression level in budding yeast by manipulating its copy number in the genome. Using this method, we construct various...

詳細記述

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書誌詳細
出版年:STAR Protoc
主要な著者: Qu, Yimiao, Jiang, Jun, Yang, Xiaojing, Tang, Chao
フォーマット: Artigo
言語:Inglês
出版事項: Elsevier 2020
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580245/
https://ncbi.nlm.nih.gov/pubmed/33111115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100082
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