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Protocol for Titrating Gene Expression Levels in Budding Yeast

The biological phenotype is affected by the level of gene expression. Here, we provide a step-by-step protocol for precisely titrating and quantitatively observing the target gene expression level in budding yeast by manipulating its copy number in the genome. Using this method, we construct various...

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Bibliografische gegevens
Gepubliceerd in:STAR Protoc
Hoofdauteurs: Qu, Yimiao, Jiang, Jun, Yang, Xiaojing, Tang, Chao
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: Elsevier 2020
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580245/
https://ncbi.nlm.nih.gov/pubmed/33111115
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100082
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