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Protocol for measuring sphingolipid metabolism in budding yeast

Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceram...

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Enregistré dans:
Détails bibliographiques
Publié dans:STAR Protoc
Auteurs principaux: Ikeda, Atsuko, Hanaoka, Kazuki, Funato, Kouichi
Format: Artigo
Langue:Inglês
Publié: Elsevier 2021
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC8063906/
https://ncbi.nlm.nih.gov/pubmed/33912844
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100412
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