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Protocol for measuring sphingolipid metabolism in budding yeast
Sphingolipid biosynthesis occurs in both the endoplasmic reticulum (ER) and the Golgi apparatus. Ceramide synthesized in the ER is transported to the Golgi and incorporated into complex sphingolipids. Here, we present a step-by-step protocol to analyze sphingolipid metabolism in budding yeast. Ceram...
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| Publié dans: | STAR Protoc |
|---|---|
| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Elsevier
2021
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC8063906/ https://ncbi.nlm.nih.gov/pubmed/33912844 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2021.100412 |
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