Convenient Protocol for Production and Purification of Clostridioides difficile Spores for Germination Studies

Clostridioides difficile, an obligate anaerobic bacterium, causes infections leading to prolonged diarrhea. The bacterium produces dormant spores that can withstand an aerobic environment, resulting in easy environmental transfer. Here, we present a convenient sporulation and purification protocol t...

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Bibliographic Details
Published in:STAR Protoc
Main Authors: Weldy, Melissa, Evert, Clayton, Dosa, Peter I., Khoruts, Alexander, Sadowsky, Michael J.
Format: Artigo
Language:Inglês
Published: Elsevier 2020
Subjects:
Protocol
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580112/
https://ncbi.nlm.nih.gov/pubmed/33111107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100071
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