Convenient Protocol for Production and Purification of Clostridioides difficile Spores for Germination Studies

Clostridioides difficile, an obligate anaerobic bacterium, causes infections leading to prolonged diarrhea. The bacterium produces dormant spores that can withstand an aerobic environment, resulting in easy environmental transfer. Here, we present a convenient sporulation and purification protocol t...

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Bibliografiske detaljer
Udgivet i:STAR Protoc
Main Authors: Weldy, Melissa, Evert, Clayton, Dosa, Peter I., Khoruts, Alexander, Sadowsky, Michael J.
Format: Artigo
Sprog:Inglês
Udgivet: Elsevier 2020
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Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC7580112/
https://ncbi.nlm.nih.gov/pubmed/33111107
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.xpro.2020.100071
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