Electroporation and genetic supply of Cas9 increase the generation efficiency of CRISPR/Cas9 knock-in alleles in C57BL/6J mouse zygotes

CRISPR/Cas9 machinery delivered as ribonucleoprotein (RNP) to the zygote has become a standard tool for the development of genetically modified mouse models. In recent years, a number of reports have demonstrated the effective delivery of CRISPR/Cas9 machinery via zygote electroporation as an altern...

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Bibliographic Details
Published in:Sci Rep
Main Authors: Alghadban, Samy, Bouchareb, Amine, Hinch, Robert, Hernandez-Pliego, Polinka, Biggs, Daniel, Preece, Chris, Davies, Benjamin
Format: Artigo
Language:Inglês
Published: Nature Publishing Group UK 2020
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC7578782/
https://ncbi.nlm.nih.gov/pubmed/33087834
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/s41598-020-74960-7
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