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Increasing knock-in efficiency in mouse zygotes by transient hypothermia
Integration of a point mutation to correct or edit a gene requires the repair of the CRISPR-Cas9-induced double-strand break by homology directed repair (HDR). This repair pathway is more active in late S and G2 phases of the cell cycle, whereas the competing pathway of non-homologous end joining (N...
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| Publicado no: | CRISPR J |
|---|---|
| Main Authors: | , , , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2024
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7615915/ https://ncbi.nlm.nih.gov/pubmed/38635329 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1089/crispr.2023.0077 |
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